References/Publications for ImmunoCAP Test of Fag e 1

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11097313 Allergy. 2000 Nov;55(11):1035-41.

Identification and characterization of the major allergens of buckwheat.

Park JW(1), Kang DB, Kim CW, koh SH, Yum HY, Kim KE, Hong CS, Lee KY.

21461893 Arch Dermatol Res. 2011 Nov;303(9):635-42. doi: 10.1007/s00403-011-1142-z. Epub
2011 Apr 3.

Usability of Fag e 2 ImmunoCAP in the diagnosis of buckwheat allergy.

Tohgi K(1), Kohno K, Takahashi H, Matsuo H, Nakayama S, Morita E.

Specific IgE antibodies were determined using the ImmunoCAP method for native
Fag e 1 and Fag e 2, recombinant Fag e 1 and Fag e 2, and crude buckwheat extract
(f11) in 10 buckwheat allergy patients, 14 atopic dermatitis patients, and 15
healthy subjects. All buckwheat allergy patients showed positive results for
native Fag e 1- and Fag e 2-specific IgE tests and for ImmunoCAP (f11). In
contrast, the rates of atopic dermatitis patients with positive results for
native Fag e 1- and Fag e 2-specific IgE tests were 64.2% (9/14) and 57.1%
(8/14), respectively. The sensitivities of the test using recombinant proteins
were lower than those of the test using native proteins. The area under the curve
(AUC) as determined by receiver operating characteristic (ROC) curve analysis was
the largest for the native Fag e 2-specific IgE test (0.967), with a sensitivity
of 90% and a specificity of 89.6% (cut-off: 2.74 kUa/L). Thus, the native Fag e
2-specific IgE antibody titer obtained using the ImmunoCAP method is more
reliable than the buckwheat ImmunoCAP (f11) value for predicting buckwheat

DOI: 10.1007/s00403-011-1142-z
PMID: 21461893 [Indexed for MEDLINE]