References/Publications for Western/Immunoblot of Der f 7

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8556554 Clin Exp Allergy. 1995 Oct;25(10):1000-6.

Molecular cloning and immunological characterization of the house dust mite
allergen Der f 7.

Shen HD(1), Chua KY, Lin WL, Hsieh KH, Thomas WR.

9249276 Clin Exp Allergy. 1997 Jul;27(7):824-32.

Characterization of the allergen Der f 7 from house dust mite extracts by
species-specific and crossreactive monoclonal antibodies.

Shen HD(1), Lin WL, Tsai LC, Tam MF, Chua KY, Chen HL, Hsieh KH, Li CS, Thomas

20567249 Immunol Cell Biol. 2011 Feb;89(2):225-30. doi: 10.1038/icb.2010.77. Epub 2010 Jun

Homology modeling and monoclonal antibody binding of the Der f 7 dust mite

Shen HD(1), Tam MF, Huang CH, Chou H, Tai HY, Chen YS, Sheu SY, Thomas WR.

20939383 J Med Entomol. 2010 Sep;47(5):868-76.

Cloning, expression, and characterization of Der f 7, an allergen of
Dermatophagoides farinae from China.

Cui YB(1), Cai HX, Zhou Y, Gao CX, Shi WH, Yu M, Li L.

21820178 Mol Immunol. 2011 Sep;48(15-16):2130-4. doi: 10.1016/j.molimm.2011.07.004. Epub
2011 Aug 5.

Asp159 is a critical core amino acid of an IgE-binding and cross-reactive epitope
of a dust mite allergen Der f 7.

Chou H(1), Tam MF, Lee SS, Tang RB, Lin TH, Tai HY, Chen YS, Shen HD.

22644529 Appl Microbiol Biotechnol. 2012 Dec;96(5):1275-82. doi:
1007/s00253-012-4169-y. Epub 2012 May 31.

Transcripts of a heterologous gene encoding mite allergen Der f 7 are stabilized
by codon optimization in Aspergillus oryzae.

Tanaka M(1), Tokuoka M, Shintani T, Gomi K.

24818391 Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2013 Oct;31(5):363-6.

[Cloning, expression and identification of Der f7 gene from Dermatophagoides
farinae and its immunological characteristics].

[Article in Chinese]

Zheng MY, Wu YL, Yan H, Ji KM, Liu ZG.

OBJECTIVE: To clone and express Der f7 gene of Dermatophagoides farinae, and
identify its immunogenicity.
METHODS: Total RNA was extracted from D. farinae mites. A reference sequence
(Accession No. AY283292) was used to design specific primers. The Der f7 gene
fragment was amplified by RT-PCR, and cloned into pET-32a vector. The recombinant
plasmid was transformed into E. coli BL21 (DE3) and induced with IPTG for protein
expression. The recombinant protein was purified by Ni2+ chelating affinity
chromatography and analyzed by SDS-PAGE and Western blotting.
RESULTS: The Der f7 gene fragment was about 650 bp, and shared 99% homology with
the published one (Accession No. FJ436108). SDS-PAGE result showed its relative
molecular weight (M(r)) of 23 000. The recombinant protein showed appropriate
combination ability with IgE in sera of mite allergic patients.
CONCLUSION: Der f 7 gene has been expressed in prokaryotic expression system and
shows allergenicity.

PMID: 24818391 [Indexed for MEDLINE]