| Select | PubMed ID | Description |
| 8556554 | Clin Exp Allergy. 1995 Oct;25(10):1000-6.
Molecular cloning and immunological characterization of the house dust mite
allergen Der f 7.
Shen HD(1), Chua KY, Lin WL, Hsieh KH, Thomas WR.
|
| 9249276 | Clin Exp Allergy. 1997 Jul;27(7):824-32.
Characterization of the allergen Der f 7 from house dust mite extracts by
species-specific and crossreactive monoclonal antibodies.
Shen HD(1), Lin WL, Tsai LC, Tam MF, Chua KY, Chen HL, Hsieh KH, Li CS, Thomas
WR.
|
| 20567249 | Immunol Cell Biol. 2011 Feb;89(2):225-30. doi: 10.1038/icb.2010.77. Epub 2010 Jun
Homology modeling and monoclonal antibody binding of the Der f 7 dust mite
allergen.
Shen HD(1), Tam MF, Huang CH, Chou H, Tai HY, Chen YS, Sheu SY, Thomas WR.
|
| 20939383 | J Med Entomol. 2010 Sep;47(5):868-76.
Cloning, expression, and characterization of Der f 7, an allergen of
Dermatophagoides farinae from China.
Cui YB(1), Cai HX, Zhou Y, Gao CX, Shi WH, Yu M, Li L.
|
| 21820178 | Mol Immunol. 2011 Sep;48(15-16):2130-4. doi: 10.1016/j.molimm.2011.07.004. Epub
2011 Aug 5.
Asp159 is a critical core amino acid of an IgE-binding and cross-reactive epitope
of a dust mite allergen Der f 7.
Chou H(1), Tam MF, Lee SS, Tang RB, Lin TH, Tai HY, Chen YS, Shen HD.
|
| 22644529 | Appl Microbiol Biotechnol. 2012 Dec;96(5):1275-82. doi:
1007/s00253-012-4169-y. Epub 2012 May 31.
Transcripts of a heterologous gene encoding mite allergen Der f 7 are stabilized
by codon optimization in Aspergillus oryzae.
Tanaka M(1), Tokuoka M, Shintani T, Gomi K.
|
| 24818391 | Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2013 Oct;31(5):363-6.
[Cloning, expression and identification of Der f7 gene from Dermatophagoides
farinae and its immunological characteristics].
[Article in Chinese]
Zheng MY, Wu YL, Yan H, Ji KM, Liu ZG.
OBJECTIVE: To clone and express Der f7 gene of Dermatophagoides farinae, and
identify its immunogenicity.
METHODS: Total RNA was extracted from D. farinae mites. A reference sequence
(Accession No. AY283292) was used to design specific primers. The Der f7 gene
fragment was amplified by RT-PCR, and cloned into pET-32a vector. The recombinant
plasmid was transformed into E. coli BL21 (DE3) and induced with IPTG for protein
expression. The recombinant protein was purified by Ni2+ chelating affinity
chromatography and analyzed by SDS-PAGE and Western blotting.
RESULTS: The Der f7 gene fragment was about 650 bp, and shared 99% homology with
the published one (Accession No. FJ436108). SDS-PAGE result showed its relative
molecular weight (M(r)) of 23 000. The recombinant protein showed appropriate
combination ability with IgE in sera of mite allergic patients.
CONCLUSION: Der f 7 gene has been expressed in prokaryotic expression system and
shows allergenicity.
PMID: 24818391 [Indexed for MEDLINE]
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