| 22139179 | Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Dec 1;67(Pt 12):1612-5.
doi: 10.1107/S174430911103836X. Epub 2011 Nov 26.
Cloning, expression, purification, crystallization and preliminary X-ray
diffraction studies of a major group 7 allergen, Der f 7, from the dust mite
Dermatophagoides farinae.
Tan KW(1), Kumar S, Chew FT, Mok YK.
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| 24818391 | Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2013 Oct;31(5):363-6.
[Cloning, expression and identification of Der f7 gene from Dermatophagoides
farinae and its immunological characteristics].
[Article in Chinese]
Zheng MY, Wu YL, Yan H, Ji KM, Liu ZG.
OBJECTIVE: To clone and express Der f7 gene of Dermatophagoides farinae, and
identify its immunogenicity.
METHODS: Total RNA was extracted from D. farinae mites. A reference sequence
(Accession No. AY283292) was used to design specific primers. The Der f7 gene
fragment was amplified by RT-PCR, and cloned into pET-32a vector. The recombinant
plasmid was transformed into E. coli BL21 (DE3) and induced with IPTG for protein
expression. The recombinant protein was purified by Ni2+ chelating affinity
chromatography and analyzed by SDS-PAGE and Western blotting.
RESULTS: The Der f7 gene fragment was about 650 bp, and shared 99% homology with
the published one (Accession No. FJ436108). SDS-PAGE result showed its relative
molecular weight (M(r)) of 23 000. The recombinant protein showed appropriate
combination ability with IgE in sera of mite allergic patients.
CONCLUSION: Der f 7 gene has been expressed in prokaryotic expression system and
shows allergenicity.
PMID: 24818391 [Indexed for MEDLINE]
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