References/Publications for ELISA of Ani s 3

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25668895 J Investig Allergol Clin Immunol. 2014;24(6):431-8.

In vivo and in vitro testing with rAni s 1 can facilitate diagnosis of Anisakis
simplex allergy.

Martínez-Aranguren RM, Gamboa PM, García-Lirio E, Asturias J, Goikoetxea MJ, Sanz

BACKGROUND: Traditional diagnostic tests such as skin prick tests (SPT) and
specific IgE (slgE) against whole Anisakis simplex extract have low specificity.
Consequently, allergy to A simplex is overdiagnosed.
OBJECTIVE: Our aim was to compare tests used in component-resolved diagnosis.
METHODS: We evaluated 34 patients with allergy to A simplex, 15 patients with
acute urticaria who were sensitized to A simplex but had no clinical history of
allergy to A simplex, and 10 patients allergic to seafood. SPT, slgE (ELISA and
ISAC-I 12), and the basophil activation test (BAT) were performed with A simplex
whole extract and the molecular components rAni s 1, rAni s 3, and nPen m 1.
Sensitivity and specificity were calculated and compared with different cutoffs.
RESULTS: With the A simplex whole extract, SPT, slgE, and BAT yielded specificity
values of 72%, 68%, and 70%, respectively, with a cutoff (wheal size) of 11.2 mm,
an slgE value of 7.9 kUAIL, and a stimulation index of 1.9. Specificity increased
to 100% using the molecular component rAni s 1 with SPT, slgE by ELISA, and
ISAC-112. Neither rAni s 3 sensitization nor cross-reactivity with Pen m 1 was
observed in patients sensitized to A simplex.
CONCLUSION: rAni s 1 is recognized by 100% of our patients and is able to
distinguish between patients allergic to A simplex and patients with acute
urticaria who are sensitized to A simplex but have no clinical history of allergy
to this parasite.

PMID: 25668895 [Indexed for MEDLINE]