AllerBase Allergen Database

Data for Bla g 2

WHO/IUIS recognized Allergen

1 1 1 1 1 1 1 1 1

Completeness Index: Denotes availibility of data for the allergen in Binary format
1: data available
0: data not available

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AllerBase ID	AB_A_00305	Allergen Name	Bla g 2
Source Organism	Blattella germanica (German cockroach)	Kingdom	Animal
Biological Function/Role	Aspartic protease	Mode of Exposure	Inhalation

Assays/Methods used for Experimental Characterization (Read More)

: Data Available

: Data not Available

Biological assay / test	IgE assay / test	Physicochemical test	Cell-based assay
Basophil/Mast cell/Histamine Test (Reference/s)	EAST	Chromatography (Reference/s)	Lymphocyte proliferation assay (Reference/s)
Bronchial Test	ELISA (Reference/s)	Isoelectrofocusing
Conjunctival Test	Crossed immunoelectrophoresis	Mass spectrometry (MS) (Reference/s)
Nasal Test	Fluorescence Immunoassay	N-terminal sequencing/sequence analysis (Reference/s)
Oral Test	Immunochemiluminescent assay
Passive Cutaneous Anaphylaxis (PCA)	ImmunoCAP Test (Reference/s)
Skin Test (Reference/s)	Microarray (Reference/s)
	Radio Immunoassay (Reference/s)
	RAST (Reference/s)
	Western/Immunoblot (Reference/s)

Isoallergen/Variant for Bla g 2:

Available

Isoallergen/Variant	Common Name	GenBank Nucleotide	NCBI Protein (GenPept)	UniProtKB	RCSB-PDB (Click on the PDB id to view structure)	ETR (Evolutionary Trace analysis Report)
Bla g 2.0101	Aspartic protease Bla g 2	U28863.1 (View Sequence)	AAA86744.1 (View Sequence)	P54958 (View Sequence)	1YG9 3LIZ 2NR6 4RLD	1yg9 3liz 2nr6 4rld

Sequence Analysis

BLAST
Pairwise alignment
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BLAST Nucleotide/Protein sequence of Allergen Bla g 2 with Nucleotide or Protein sequences in AllerBase:
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Cross-links to Allergen Databases

IUIS	IUIS-Allergen
Allergome	141
AllFam	AF004

IgE Epitope Data

Allergen

Isoallergen / Isoform

Epitope

Type of Epitope

Sequence Position

View on Structure

IEDB

Antibody Used

IMGT

AgAbDb Link

Nature of Epitope

Assay

Reference

Comment

Bla g 2

Bla g 2.0101

85KPKYISDGN93;107RGIE110;122QQD124;153NALK156

Discontinuous/ Conformational

85-93;107-110;122-124;153-156

1YG9 3LIZ 2NR6
4RLD

98668

Monoclonal antibody 7C11

IMGT/3Dstructure-DB

AgAbDb

Epitope on Bla g 2 homodimer

Allergen-Antibody Complex Structure

18519566

Bla g 2

Bla g 2.0101

224IGDT227;249KAYVNPINEAI259;275DCSKIPSLPDVT286;295NISSQ299

Discontinuous/ Conformational

224-227;249-259;275-286;295-299

1YG9 3LIZ 2NR6
4RLD

143264

Monoclonal antibody 4C3

IMGT/3Dstructure-DB

AgAbDb

Allergen-Antibody Complex Structure, ELISA

21789239

Bla g 2

Bla g 2.0101

K156;F187;K278

Discontinuous/ Conformational

156;187;278

1YG9 3LIZ 2NR6
4RLD

Site-directed mutagenesis, Structural analysis, ELISA

26644466

IgE Antibody Data

Antibody data

Allergen data

Antibody/ Chain Description	Clone/ Isolate	Antibody Source	IMGT	NCBI Protein (GenPept)	Broad Allergen Category	Allergen Id /Name	Material used for Sensitization	Allergic Disease	Antibody studied in	Reference
Fab light chain	PDB:2NR6	Mus musculus	-	-	Respiratory	Bla g 2	rBla g 2	Not specified	Not specified	18519566
Fab heavy chain	PDB:2NR6	Mus musculus	-	-	Respiratory	Bla g 2	rBla g 2	Not specified	Not specified	18519566
4C3 mAb light chain	PDB:3LIZ	Mus musculus	-	-	Respiratory	Bla g 2	rBla g 2	Not specified	Not specified	21789239
4C3 mAb heavy chain	PDB:3LIZ	Mus musculus	-	-	Respiratory	Bla g 2	rBla g 2	Not specified	Not specified	21789239

Cross-reactivity Data

Allergen	Cross-reactive Allergens	Reference
Bla g 2	Rhi o 1 (View AllerBase Entry)	26672984