AllerBase Allergen Database

Data for Api m 2

WHO/IUIS recognized Allergen

1 1 1 1 1 1 1 1 1

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AllerBase ID	AB_A_00154	Allergen Name	Api m 2
Source Organism	Apis mellifera (Honey bee)	Kingdom	Animal
Biological Function/Role	Hyaluronidase	Mode of Exposure	Sting

Assays/Methods used for Experimental Characterization (Read More)

: Data Available

: Data not Available

Biological assay / test	IgE assay / test	Physicochemical test	Cell-based assay
Basophil/Mast cell/Histamine Test (Reference/s)	EAST	Chromatography (Reference/s)	Lymphocyte proliferation assay (Reference/s)
Bronchial Test	ELISA (Reference/s)	Isoelectrofocusing
Conjunctival Test	Crossed immunoelectrophoresis	Mass spectrometry (MS)
Nasal Test	Fluorescence Immunoassay	N-terminal sequencing/sequence analysis
Oral Test	Immunochemiluminescent assay
Passive Cutaneous Anaphylaxis (PCA)	ImmunoCAP Test (Reference/s)
Skin Test (Reference/s)	Microarray
	Radio Immunoassay
	RAST (Reference/s)
	Western/Immunoblot (Reference/s)

Isoallergen/Variant for Api m 2:

Available

Isoallergen/Variant	Common Name	GenBank Nucleotide	NCBI Protein (GenPept)	UniProtKB	RCSB-PDB (Click on the PDB id to view structure)	ETR (Evolutionary Trace analysis Report)
Api m 2.0101	Hyaluronidase	L10710.1 (View Sequence)	AAA27730.1 (View Sequence)	Q08169 (View Sequence)	1FCQ 1FCU 1FCV 2J88	1fcq 1fcu 1fcv 2j88

Sequence Analysis

BLAST
Pairwise alignment
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BLAST Nucleotide/Protein sequence of Allergen Api m 2 with Nucleotide or Protein sequences in AllerBase:
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Cross-links to Allergen Databases

IUIS	IUIS-Allergen
Allergome	46
AllFam	AF103

IgE Epitope Data

Allergen

Isoallergen / Isoform

Epitope

Type of Epitope

Sequence Position

View on Structure

IEDB

Antibody Used

IMGT

AgAbDb Link

Nature of Epitope

Assay

Reference

Comment

Api m 2

Api m 2.0101

R170;173HPFWDDQR180

Discontinuous/ Conformational

170;173-180

1FCQ 1FCU 1FCV
2J88

77406

Monoclonal antibody 21E11

IMGT/3Dstructure-DB

AgAbDb

Allergen-Antibody Complex Structure

17374540

IgE Antibody Data

Antibody data

Allergen data

Antibody/ Chain Description	Clone/ Isolate	Antibody Source	IMGT	NCBI Protein (GenPept)	Broad Allergen Category	Allergen Id /Name	Material used for Sensitization	Allergic Disease	Antibody studied in	Reference
Fab light chain	PDB:2J88	Mus musculus	-	-	Contact	Api m 2	Recombinant Hyaluronidase	Not specified	Not specified	17374540
Fab heavy chain	PDB:2J88	Mus musculus	-	-	Contact	Api m 2	Recombinant Hyaluronidase	Not specified	Not specified	17374540

Cross-reactivity Data

Allergen	Cross-reactive Allergens	Reference
Api m 2	Api d Hyal (View AllerBase Entry)	28712914
Api m 2	Dol m 2 (View AllerBase Entry)	7876212