References/Publications for ELISA of Poa p 1

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2434551 J Allergy Clin Immunol. 1987 Mar;79(3):489-95.

Cross-reactive and unique grass group I antigenic determinants defined by
monoclonal antibodies.

Esch RE, Klapper DG.

As part of a study to probe the immunochemical basis for allergenic
cross-reactivity among grass pollens, a series of useful reagents has been
prepared. The major grass-pollen allergen, designated group I (GpI), was isolated
from five grass pollens (meadow fescue, June grass, sweet vernal grass, redtop
grass, and perennial ryegrass). The purified GpI antigens were used to immunize
individual groups of BALB/c mice. A total of 123 hybridoma-derived anti-GpI
monoclonal antibodies (mAbs) was produced. These mAbs were used to evaluate the
antigenic relationship among the GpI antigens by means of two types of ELISA. The
experiments revealed a high level of epitope diversity and demonstrated a wide
range of antibody specificities. A cross-reactivity ELISA was used to identify
and compare antigenic determinants on the GpI molecules, and it was possible to
define mAbs with specificities unique for the immunizing allergen and other mAbs
that cross-reacted with one or more other members of the test panel of allergens.
These murine mAbs reflect the range of specificities present in sera from
grass-allergic individuals.


PMID: 2434551 [Indexed for MEDLINE]
7515604
Ann Allergy. 1994 Jun;72(6):499-506.

Recombinant allergens and diagnosis of grass pollen allergy.

Olsen E(1), Mohapatra SS.


18004069 Int Arch Allergy Immunol. 2008;145(4):283-90. Epub 2007 Nov 13.

Antigenic similarity among group 1 allergens from grasses and quantitation ELISA
using monoclonal antibodies to Phl p 1.

Duffort O(1), Quintana J, Ipsen H, Barber D, Polo F.


19895591 Clin Exp Allergy. 2010 Mar;40(3):505-19. doi: 10.1111/j.1365-2222.2009.03380.x.
Epub 2009 Nov 5.

Molecular variability of group 1 and 5 grass pollen allergens between Pooideae
species: implications for immunotherapy.

Chabre H(1), Gouyon B, Huet A, Baron-Bodo V, Nony E, Hrabina M, Fenaille F,
Lautrette A, Bonvalet M, Maillère B, Bordas-Le Floch V, Van Overtvelt L, Jain K,
Ezan E, Batard T, Moingeon P.