References/Publications for Skin Test of Pho d 2

Check all              

Select PubMed ID Description
15784118 Clin Exp Allergy. 2005 Mar;35(3):374-81.

Pho d 2, a major allergen from date palm pollen, is a profilin: cloning,
sequencing, and immunoglobulin E cross-reactivity with other profilins.

Asturias JA(1), Ibarrola I, Fernández J, Arilla MC, González-Rioja R, Martínez A.

18489029 Clin Exp Allergy. 2008 Jun;38(6):1033-7. doi: 10.1111/j.1365-2222.2008.02980.x.
Epub 2008 Apr 13.

Profilin sensitization detected in the office by skin prick test: a study of
prevalence and clinical relevance of profilin as a plant food allergen.

Asero R(1), Monsalve R, Barber D.

All were sensitized to grass pollen, and most of them reacted to birch,
mugwort, ragweed and plantain pollen as well. SPT with pellitory and cypress
scored negative in a high proportion of profilin reactors [26/60 (43%) and 33/60
(55%), respectively]. More than one half (34/60 [57%]) of profilin reactors had
food allergy; 21 of these were monosensitized to profilin, 11 were sensitized to
both profilin and Bet v 1 homologous protein, one to both profilin and LTP, and
one to all the three allergens. The large majority of profilin-allergic patients
reported oral allergy syndrome as the only food-induced symptom and were able to
tolerate the offending foods if they were cooked or otherwise processed.
Twenty-eight of 34 reported reactivity to two or more plant-derived foods.
Rosaceae, tree nuts, melon and watermelon, tomato, pineapple, citrus fruits and
banana were the more frequently offending foods.
CONCLUSION: Profilin should be considered a clinically relevant food allergen.
Allergy to melon, watermelon, tomato, banana, pineapple and orange may be
considered as a marker of profilin hypersensitivity. This study underlines the
clinical importance of being able to diagnose hypersensitivity to single food
allergenic proteins by SPT, particularly when the relevant food allergen sources
contain several allergens that show different chemical/physical features and,
hence, completely different risk profiles.

DOI: 10.1111/j.1365-2222.2008.02980.x
PMID: 18489029 [Indexed for MEDLINE]
Eur Ann Allergy Clin Immunol. 2008 Dec;40(4):115-21.

Component-resolved diagnosis of plant food allergy by SPT.

Asero R(1), Jimeno L, Barber D.

20232772 J Investig Allergol Clin Immunol. 2010;20(1):35-8.

Preliminary results of a skin prick test-based study of the prevalence and
clinical impact of hypersensitivity to pollen panallergens (polcalcin and

Asero R(1), Jimeno L, Barber D.

26310043 J Investig Allergol Clin Immunol. 2015;25(4):283-7.

Do Skin Prick Test and In Vitro Techniques Diagnose Sensitization to Peach Lipid
Transfer Protein and Profilin Equally Well in Allergy to Plant Food and Pollen?

Goikoetxea MJ, Berroa F, Cabrera-Freitag P, Ferrer M, Núñez-Córdoba JM, Sanz ML,
Gastaminza G.

OBJECTIVE: To compare the skin prick test (SPT) with in vitro techniques (single
and multiplex fluorescence enzyme-immunoassay [FEIA]) for detecting sensitization
to profilin and lipid transfer protein (LTP).
METHODS: We retrospectively studied 181 patients with pollen and/or plant food
allergy and 61 controls. SPT was performed with date palm profilin (Pho d 2) and
peach LTP (Pru p 3), and specific IgE (sIgE) to Phl p 12 and Pru p 3 was analyzed
using single FEIA and microarray.
RESULTS: Fifteen of 201 patients with negative results for LTP in the SPT were
sensitized to this allergen in the in vitro tests, and 18 of 41 patients with
positive results for LTP in the SPT were not sensitized according to the in vitro
tests. Seventeen of 186 patients with negative results for profilin in the SPT
were sensitized to Phl p 12 by serum sIgE, and 30 out of 56 patients with
positive results for profilin in SPT were not sensitized to Phl p 12 according to
the other tests. Moderate agreement was observed between the 3 techniques
CONCLUSIONS: SPT is a sensitive technique for detecting sensitization to LTP and
profilin. Its results are similar to those of in vitro techniques, especially in
patients with negative SPT results for peach LTP and palm tree profilin.

PMID: 26310043 [Indexed for MEDLINE]