References/Publications for Western/Immunoblot of Phl p 5

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1643437 Int Arch Allergy Immunol. 1992;98(2):105-9.

Characterization of isoforms of the major allergen Phl p V by two-dimensional
immunoblotting and microsequencing.

Petersen A(1), Becker WM, Schlaak M.

1863892 Clin Exp Allergy. 1991 May;21(3):297-307.

Group V allergens in grass pollens. I. Purification and characterization of the
group V allergen from Phleum pratense pollen, Phl p V.

Matthiesen F(1), Løwenstein H.

7516814 Clin Exp Allergy. 1994 Mar;24(3):250-6.

Epitope analysis of isoforms of the major allergen Phl p V by fingerprinting and

Petersen A(1), Becker WM, Schlaak M.

7520460 J Allergy Clin Immunol. 1994 Aug;94(2 Pt 1):173-81.

Major allergen Phl p Va (timothy grass) bears at least two different IgE-reactive

Bufe A(1), Becker WM, Schramm G, Petersen A, Mamat U, Schlaak M.

7542075 Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):242-4.

Molecular characterization of timothy grass pollen group V allergens.

Becker WM(1), Bufe A, Petersen A, Schlaak M.

7544180 Int Arch Allergy Immunol. 1995 Sep;108(1):49-54.

Characterization of the allergen group VI in timothy grass pollen (Phl p 6). I.
Immunological and biochemical studies.

Petersen A(1), Bufe A, Schlaak M, Becker WM.

7982125 Clin Exp Allergy. 1994 Aug;24(8):752-7.

A two-site monoclonal antibody ELISA for the quantification of group V allergens
in grass extracts.

Fahlbusch B(1), Schlenvoigt G, Müller WD, Weber B, Jäger L.

8359821 Hybridoma. 1993 Jun;12(3):249-58.

Successful technique for the selective production of monoclonal antibodies
against a major allergenic component in timothy pollen extract.

Petersen A(1), Becker WM, Schlaak M.

8613635 J Allergy Clin Immunol. 1996 Mar;97(3):781-7.

Immunologic characterization of purified recombinant timothy grass pollen (Phleum
pratense) allergens (Phl p 1, Phl p2, Phl p 5).

Vrtala S(1), Susani M, Sperr WR, Valent P, Laffer S, Dolecek C, Kraft D, Valenta

8828543 J Allergy Clin Immunol. 1996 Sep;98(3):652-8.

Comparison of recombinant timothy grass pollen allergens with natural extract for
diagnosis of grass pollen allergy in different populations.

Laffer S(1), Spitzauer S, Susani M, Pairleitner H, Schweiger C, Grönlund H, Menz
G, Pauli G, Ishii T, Nolte H, Ebner C, Sehon AH, Kraft D, Eichler HG, Valenta R.

9973522 J Immunol. 1999 Feb 15;162(4):2406-14.

"Allergen engineering": variants of the timothy grass pollen allergen Phl p 5b
with reduced IgE-binding capacity but conserved T cell reactivity.

Schramm G(1), Kahlert H, Suck R, Weber B, Stüwe HT, Müller WD, Bufe A, Becker WM,
Schlaak MW, Jäger L, Cromwell O, Fiebig H.

10224462 Int Arch Allergy Immunol. 1999 Feb-Apr;118(2-4):414-8.

Timothy grass (Phleum pratense L.) pollen as allergen carriers and initiators of
an allergic response.

Behrendt H(1), Tomczok J, Sliwa-Tomczok W, Kasche A, Ebner von Eschenbach C,
Becker WM, Ring J.

10231323 Clin Exp Allergy. 1999 May;29(5):633-41.

Concentrations of major grass group 5 allergens in pollen grains and atmospheric
particles: implications for hay fever and allergic asthma sufferers sensitized to
grass pollen allergens.

Schäppi GF(1), Taylor PE, Pain MC, Cameron PA, Dent AW, Staff IA, Suphioglu C.

12752597 Clin Exp Allergy. 2003 May;33(5):669-75.

Characterization of immunoglobulin E responses in Balb/c mice against the major
allergens of timothy grass (Phleum pratense) pollen.

Seitzer U(1), Bussler H, Kullmann B, Petersen A, Becker WM, Ahmed J.

19385952 Allergy. 2009 Oct;64(10):1486-92. doi: 10.1111/j.1398-9995.2009.02040.x. Epub
2009 Apr 6.

Allergen content of grass pollen preparations for skin prick testing and
sublingual immunotherapy.

Sander I(1), Fleischer C, Meurer U, Brüning T, Raulf-Heimsoth M.

21143538 Clin Exp Allergy. 2011 Feb;41(2):270-80. doi: 10.1111/j.1365-2222.2010.03666.x.
Epub 2010 Dec 10.

Recombinant monoclonal human immunoglobulin E to investigate the allergenic
activity of major grass pollen allergen Phl p 5.

Madritsch C(1), Flicker S, Scheiblhofer S, Zafred D, Pavkov-Keller T, Thalhamer
J, Keller W, Valenta R.

21474184 Mol Immunol. 2011 May;48(9-10):1236-44. doi: 10.1016/j.molimm.2011.03.005. Epub
2011 Apr 6.

Generation and epitope analysis of human monoclonal antibody isotypes with
specificity for the Timothy grass major allergen Phl p 5a.

Hecker J(1), Diethers A, Etzold S, Seismann H, Michel Y, Plum M, Bredehorst R,
Blank S, Braren I, Spillner E.

21720171 Int Arch Allergy Immunol. 2011;156(3):259-66. doi: 10.1159/000323733. Epub 2011
Jun 29.

Expression of a major plant allergen as membrane-anchored and secreted protein in
human cells with preserved T cell and B cell epitopes.

Baranyi U(1), Gattringer M, Boehm A, Marth K, Focke-Tejkl M, Bohle B, Blatt K,
Valent P, Valenta R, Wekerle T.

28532654 J Allergy Clin Immunol. 2017 Oct;140(4):1187-1191. doi:
1016/j.jaci.2017.05.005. Epub 2017 May 19.

Flexible IgE epitope-containing domains of Phl p 5 cause high allergenic

Göbl C(1), Focke-Tejkl M(2), Najafi N(2), Schrank E(3), Madl T(4), Kosol S(3),
Madritsch C(2), Dorofeeva Y(2), Flicker S(2), Thalhamer J(5), Valenta R(6),
Zangger K(7), Tjandra N(8).

32375962 Allergy Asthma Proc. 2020 May 1;41(3):183-191. doi: 10.2500/aap.2020.41.200002.

Serum immunoglobulin E reactivity to cross-reacting panallergen components in
north-eastern Poland patients pollen sensitized.

Kowal K(1), Pampuch A(2), Sacharzewska E(1), Swiebocka E(3), Siergiejko Z(4),
Siergiejko G(5), Chruszcz M(6), DuBuske L(7).