|Select ||PubMed ID ||Description |
|8806590|| Biochem Biophys Res Commun. 1996 Sep 4;226(1):42-50.|
High-level expression in Escherichia coli and purification of recombinant plant
profilins: comparison of IgE-binding capacity and allergenic activity.
Vrtala S(1), Wiedemann P, Mittermann I, Eichler HG, Sperr WR, Valent P, Kraft D,
|23266139|| Allergol Immunopathol (Madr). 2014 Mar-Apr;42(2):127-35. doi:|
1016/j.aller.2012.09.001. Epub 2012 Dec 21.
Diagnostic usefulness of component-resolved diagnosis by skin prick tests and
specific IgE to single allergen components in children with allergy to fruits and
Vieira T(1), Cunha L(2), Neves E(3), Falcão H(2).
|26310043|| J Investig Allergol Clin Immunol. 2015;25(4):283-7.|
Do Skin Prick Test and In Vitro Techniques Diagnose Sensitization to Peach Lipid
Transfer Protein and Profilin Equally Well in Allergy to Plant Food and Pollen?
Goikoetxea MJ, Berroa F, Cabrera-Freitag P, Ferrer M, Núñez-Córdoba JM, Sanz ML,
OBJECTIVE: To compare the skin prick test (SPT) with in vitro techniques (single
and multiplex fluorescence enzyme-immunoassay [FEIA]) for detecting sensitization
to profilin and lipid transfer protein (LTP).
METHODS: We retrospectively studied 181 patients with pollen and/or plant food
allergy and 61 controls. SPT was performed with date palm profilin (Pho d 2) and
peach LTP (Pru p 3), and specific IgE (sIgE) to Phl p 12 and Pru p 3 was analyzed
using single FEIA and microarray.
RESULTS: Fifteen of 201 patients with negative results for LTP in the SPT were
sensitized to this allergen in the in vitro tests, and 18 of 41 patients with
positive results for LTP in the SPT were not sensitized according to the in vitro
tests. Seventeen of 186 patients with negative results for profilin in the SPT
were sensitized to Phl p 12 by serum sIgE, and 30 out of 56 patients with
positive results for profilin in SPT were not sensitized to Phl p 12 according to
the other tests. Moderate agreement was observed between the 3 techniques
CONCLUSIONS: SPT is a sensitive technique for detecting sensitization to LTP and
profilin. Its results are similar to those of in vitro techniques, especially in
patients with negative SPT results for peach LTP and palm tree profilin.
PMID: 26310043 [Indexed for MEDLINE]