References/Publications for Crossed immunoelectrophoresis of Fel d 1

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7264307 J Immunol. 1981 Sep;127(3):972-5.

Allergenically active components of cat allergen extracts.

Anderson MC, Baer H.

The allergens involved in cat allergy have been studied in pelt extracts, saliva,
serum, and urine. Using crossed immunoelectrophoresis (CIE) to examine the
antigenic content, and crossed radioimmunoelectrophoresis (CRIE) and RAST to
examine the allergenic content, it was found that allergen 1 of Dr. Ohman is the
most important allergenic component, whereas albumin and several unidentified
proteins play a minor role. Allergen 1 was not detectable in serum and urine. The
allergenic and nonallergenic proteins of pelt extract and saliva were identical
by CIE, suggesting that pelt extract proteins are mainly of salivary origin.


PMID: 7264307 [Indexed for MEDLINE]
3619132
Ann Allergy. 1987 Aug;59(2):131-4.

Identification of dander-specific and serum-specific allergens in cat dandruff
extract.

Wallenbeck I, Einarsson R.

Cat dandruff extract was characterized by crossed immunoelectrophoresis (CIE) and
crossed radioimmunoelectrophoresis (CRIE) using sera from 27 cat-sensitive
individuals. By applying a CIE/CRIE system with intermediate gel, the content of
dander-specific and serum-specific allergens was established. Thirteen
serum-related and eight dander-related antigens were identified in cat dandruff.
Of these, eight antigens were visibly radiostained in CRIE. Five allergens were
serum-specific and of these allergens cat albumin was the most important
(intermediate allergen). Three allergens were dander-specific and of these three
only cat allergen I fulfilled the criteria for a major allergen.


PMID: 3619132 [Indexed for MEDLINE]
1712068
Mol Immunol. 1991 Apr-May;28(4-5):301-9.

Studies on the biochemical structure of the major cat allergen Felis domesticus
I.

Duffort OA(1), Carreira J, Nitti G, Polo F, Lombardero M.


8156446 Clin Exp Allergy. 1994 Jan;24(1):46-52.

Bronchial provocation with cat allergen: correlation between the individual
IgE-CRIE pattern and the occurrence of a late allergic reaction.

Mosimann B(1), Aubert V, Frey JG, Leuenberger P, Pécoud A.