|Select ||PubMed ID ||Description |
|2428875|| J Immunol. 1986 Nov 1;137(9):2841-7.|
Antigen Der f I from the dust mite Dermatophagoides farinae: structural
comparison with Der p I from Dermatophagoides pteronyssinus and epitope
specificity of murine IgG and human IgE antibodies.
Heymann PW, Chapman MD, Platts-Mills TA.
The physicochemical and antigenic properties of an allergen purified from
Dermatophagoides farinae, Der f I, were compared with Der p I from
Dermatophagoides pteronyssinus. On SDS-PAGE, Der f I migrated as a single
polypeptide chain with the same m.w. as Der p I (24,000). Two isoallergenic peaks
of Der f I were identified on preparative isoelectric focusing (pI 5.7 to 6.3 and
pI 6.6 to 6.95). Fractions from each peak were shown to have an identical amino
acid composition (which was similar but not identical to Der p I) and the same
N-terminal amino acid sequence. There was a good correlation between quantitative
intradermal skin tests to both purified allergens and to D. farinae extract in
mite-allergic patients, with positive results when using as little as 10(-5)
micrograms/ml of Der f I. The majority of sera with detectable IgE antibody to D.
farinae also had IgE antibody to Der f I both among children (29/42 = 69%) and
adults (55/63 = 87%). By RAST, there was an excellent correlation between IgE
antibody to Der f I and Der p I in sera from 42 mite-allergic children (n = 0.94,
p less than 0.001). Polyclonal IgG antibodies from six mice immunized with Der f
I showed preferential binding to that allergen, and most monoclonal antibodies
(16 of 18) raised against Der f I did not bind Der p I. However, two monoclonal
antibodies from this fusion showed cross-reactive binding to both allergens.
Immunoabsorption experiments, using D. pteronyssinus and D. farinae extracts
coupled to Sepharose, showed that a large proportion of murine antibodies (74% to
Der p I and 60 to 93% to Der f I) could not be absorbed by the heterologous
extract on the immunosorbent. In contrast, in sera from seven mite-allergic
patients, most of the specific IgE and IgG antibody (i.e., greater than or equal
to 82%) was removed by either immunosorbent. Thus, Der f I and Der p I represent
a homologous pair of major allergens which possess both cross-reacting and
species-specific epitopes. The antibody response in mice immunized with either
allergen in complete Freund's adjuvant was largely directed against
species-specific epitopes, whereas in allergic humans, IgE- and IgG-specific
antibodies bound predominately to cross-reacting epitopes.
PMID: 2428875 [Indexed for MEDLINE]
Int Arch Allergy Immunol. 2002 Nov;129(3):237-41.
Association between mite allergen (Der p 1, Der f 1, Blo t 5) levels and
microscopic identification of mites or skin prick test results in asthmatic
Medeiros M Jr(1), Figueiredo JP, Almeida MC, Atta AM, Taketomi EA, Silva DA,
Terra SA, Amorim WW, Pinho RS, Araujo MI, Carvalho EM.
|12728474|| Yonsei Med J. 2003 Apr 30;44(2):313-22.|
Effect of bedding control on amount of house dust mite allergens, asthma
symptoms, and peak expiratory flow rate.
|15080832|| Allergy. 2004 May;59(5):515-9.|
Sensitization to house dust mites in Reykjavik, Iceland, in the absence of
domestic exposure to mites.
Hallas TE(1), Gislason D, Björnsdottir US, Jörundsdottir KB, Janson C, Luczynska
CM, Gislason T.
|15241351|| J Allergy Clin Immunol. 2004 Jul;114(1):105-10.|
Family history, dust mite exposure in early childhood, and risk for pediatric
atopy and asthma.
Cole Johnson C(1), Ownby DR, Havstad SL, Peterson EL.
|16689187|| J Investig Allergol Clin Immunol. 2006;16(2):129-35.|
Age, gender and reactivity to allergens independently influence skin reactivity
Bordignon V(1), Burastero SE.
|17069111|| Ann Allergy Asthma Immunol. 2006 Oct;97(4):539-45.|
Mold damage in homes and wheezing in infants.
Cho SH(1), Reponen T, LeMasters G, Levin L, Huang J, Meklin T, Ryan P, Villareal
M, Bernstein D.
|17767803|| Curr Med Res Opin. 2007 Oct;23(10):2423-31.|
A double blind, placebo-controlled cross over trial of cellulose powder by nasal
provocation with Der p1 and Der f1.
Emberlin JC(1), Lewis RA.
|28456804|| Int Arch Allergy Immunol. 2017;172(4):236-241. doi: 10.1159/000471511. Epub 2017 |
Climate and Allergic Sensitization to Airborne Allergens in the General
Population: Data from the French Six Cities Study.
Charpin D(1), Ramadour M, Lavaud F, Raherison C, Caillaud D, de Blay F, Pauli G,