References/Publications for Western/Immunoblot of Dac g 1

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1955635 J Allergy Clin Immunol. 1991 Nov;88(5):763-74.

Characterization of the major allergen of Cynodon dactylon (Bermuda grass)
pollen, Cyn d I.

Matthiesen F(1), Schumacher MJ, Løwenstein H.

2437060 Int Arch Allergy Appl Immunol. 1987;83(1):96-103.

Distribution of a major allergen of rye grass (Lolium perenne) pollen between
other grass species.

Standring R, Spackman V, Porter SJ.

Polyacrylamide gel electrophoresis in sodium dodecyl sulphate (SDS) followed by
protein staining has shown that extracts from 11 different grass pollens
contained proteins with similar molecular weights to that of the allergen R7 from
rye grass (Lolium perenne) pollen extract (i.e. 29,000-31,000 daltons). Western
blotting and detection with polyclonal (rabbit) antibodies raised against the
purified R7 (Rye I) allergen indicated that these proteins were antigenically
related and their allergenic properties were demonstrated by the binding of human
IgE to immunoblots. The distribution of cross-reacting antigenic determinants was
further investigated by immunoblotting with 2 mouse monoclonal antibodies, R7M1
and R7M2, produced with purified R7 as the initial immunogen. The 2 monoclonal
antibodies were shown to react with 'R7-like' components of grass pollen extracts
other than the component from rye grass. Differences in the distribution of R7M1
and R7M2 binding were found indicating that they are directed at separate R7

PMID: 2437060 [Indexed for MEDLINE]
J Immunol. 1988 Nov 15;141(10):3486-91.

Study of the epitope structure of purified Dac G I and Lol p I, the major
allergens of Dactylis glomerata and Lolium perenne pollens, using monoclonal

Mourad W(1), Mécheri S, Peltre G, David B, Hébert J.

2807580 Int Arch Allergy Appl Immunol. 1989;90(1):78-83.

Cloning of cDNA coding for an allergen of Cocksfoot grass (Dactylis glomerata)

Walsh DJ(1), Matthews JA, Denmeade R, Walker MR.

2913133 J Allergy Clin Immunol. 1989 Jan;83(1):144-51.

Characterization with monoclonal and polyclonal antibodies of a new major
allergen from grass pollen in the group I molecular weight range.

van Ree R(1), Clemens JG, Aalbers M, Stapel SO, Aalberse RC.

7691298 Int Arch Allergy Immunol. 1993;102(2):144-51.

Peptide mapping analysis of group I allergens of grass pollens.

Suphioglu C(1), Singh MB, Knox RB.

19895591 Clin Exp Allergy. 2010 Mar;40(3):505-19. doi: 10.1111/j.1365-2222.2009.03380.x.
Epub 2009 Nov 5.

Molecular variability of group 1 and 5 grass pollen allergens between Pooideae
species: implications for immunotherapy.

Chabre H(1), Gouyon B, Huet A, Baron-Bodo V, Nony E, Hrabina M, Fenaille F,
Lautrette A, Bonvalet M, Maillère B, Bordas-Le Floch V, Van Overtvelt L, Jain K,
Ezan E, Batard T, Moingeon P.