References/Publications for Western/Immunoblot of Bet v 1

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2807579 Int Arch Allergy Appl Immunol. 1989;90(1):54-60.

Monoclonal antibodies against birch pollen allergens: characterization by
immunoblotting and use for single-step affinity purification of the major
allergen Bet v I.

Jarolim E(1), Tejkl M, Rohac M, Schlerka G, Scheiner O, Kraft D, Breitenbach M,
Rumpold H.


1353487 Hum Immunol. 1992 Apr;33(4):259-65.

Association between IgE response against Bet v I, the major allergen of birch
pollen, and HLA-DRB alleles.

Fischer GF(1), Pickl WF, Faé I, Ebner C, Ferreira F, Breiteneder H, Vikoukal E,
Scheiner O, Kraft D.


1703514 Int Arch Allergy Appl Immunol. 1990;92(3):226-32.

Mapping of Bet v I epitopes by using murine monoclonal antibodies.

Marc-Series I(1), Boutin Y, Vrancken ER, Hébert J.


1744359 J Allergy Clin Immunol. 1991 Dec;88(6):889-94.

Recombinant allergens for immunoblot diagnosis of tree-pollen allergy.

Valenta R(1), Duchene M, Vrtala S, Birkner T, Ebner C, Hirschwehr R, Breitenbach
M, Rumpold H, Scheiner O, Kraft D.


1717531 J Allergy Clin Immunol. 1991 Oct;88(4):588-94.

Common epitopes of birch pollen and apples--studies by western and northern blot.

Ebner C(1), Birkner T, Valenta R, Rumpold H, Breitenbach M, Scheiner O, Kraft D.


7530747 J Immunol. 1995 Feb 15;154(4):1932-40.

Nonallergic individuals recognize the same T cell epitopes of Bet v 1, the major
birch pollen allergen, as atopic patients.

Ebner C(1), Schenk S, Najafian N, Siemann U, Steiner R, Fischer GW, Hoffmann K,
Szépfalusi Z, Scheiner O, Kraft D.


7545879 Allergy. 1995 Mar;50(3):215-20.

Multiplicity of cross-reactive epitopes on Bet v I as detected with monoclonal
antibodies and human IgE.

Akkerdaas JH(1), van Ree R, Aalbers M, Stapel SO, Aalberse RC.


7542069 Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):183-5.

Characterization of allergen (Bet v 1)-specific T cell lines and clones from
non-allergic individuals.

Ebner C(1), Siemann U, Najafian N, Scheiner O, Kraft D.


7549498 Int Arch Allergy Immunol. 1995 Oct;108(2):119-26.

Purification and characterization of the major allergen from apple and its
allergenic cross-reactivity with Bet v 1.

Fahlbusch B(1), Rudeschko O, Müller WD, Schlenvoigt G, Vettermann S, Jäger L.


7613152 Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):290-4.

Humoral immune responses to recombinant tree pollen allergens (Bet v I and Bet v
II) in mice: construction of a live oral allergy vaccine.

Vrtala S(1), Grote M, Ferreira F, Susani M, Stocker B, Kraft D, Valenta R.


7751517 J Allergy Clin Immunol. 1995 May;95(5 Pt 1):962-9.

Identification of allergens in fruits and vegetables: IgE cross-reactivities with
the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin).

Ebner C(1), Hirschwehr R, Bauer L, Breiteneder H, Valenta R, Ebner H, Kraft D,
Scheiner O.


7852325 J Biol Chem. 1995 Feb 10;270(6):2607-13.

Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid
chromatography, mass spectrometry, and cDNA cloning.

Swoboda I(1), Jilek A, Ferreira F, Engel E, Hoffmann-Sommergruber K, Scheiner O,
Kraft D, Breiteneder H, Pittenauer E, Schmid E, et al.


7918643 Biochim Biophys Acta. 1994 Oct 18;1219(2):457-64.

A birch gene family encoding pollen allergens and pathogenesis-related proteins.

Swoboda I(1), Scheiner O, Kraft D, Breitenbach M, Heberle-Bors E, Vicente O.


8219778 Int Arch Allergy Immunol. 1993;102(3):249-58.

Characterization of recombinant bet vI, the major pollen allergen of Betula
verrucosa (white birch), produced by fed-batch fermentation.

Larsen JN(1), Casals AB, From NB, Strøman P, Ipsen H.


8366100 J Biol Chem. 1993 Sep 15;268(26):19574-80.

Purification and characterization of recombinant Bet v I, the major birch pollen
allergen. Immunological equivalence to natural Bet v I.

Ferreira FD(1), Hoffmann-Sommergruber K, Breiteneder H, Pettenburger K, Ebner C,
Sommergruber W, Steiner R, Bohle B, Sperr WR, Valent P, et al.


8627171 J Exp Med. 1996 Feb 1;183(2):599-609.

Dissection of immunoglobulin E and T lymphocyte reactivity of isoforms of the
major birch pollen allergen Bet v 1: potential use of hypoallergenic isoforms for
immunotherapy.

Ferreira F(1), Hirtenlehner K, Jilek A, Godnik-Cvar J, Breiteneder H, Grimm R,
Hoffmann-Sommergruber K, Scheiner O, Kraft D, Breitenbach M, Rheinberger HJ,
Ebner C.


8626988 J Allergy Clin Immunol. 1996 May;97(5):1100-9.

Skin testing with recombinant allergens rBet v 1 and birch profilin, rBet v 2:
diagnostic value for birch pollen and associated allergies.

Pauli G(1), Oster JP, Deviller P, Heiss S, Bessot JC, Susani M, Ferreira F, Kraft
D, Valenta R.


8771801 Planta. 1996;199(3):413-5.

The major birch pollen allergen, Bet v 1, shows ribonuclease activity.

Bufe A(1), Spangfort MD, Kahlert H, Schlaak M, Becker WM.


8789543 Clin Exp Allergy. 1996 Jan;26(1):50-60.

Serological and skin-test diagnosis of birch pollen allergy with recombinant Bet
v I, the major birch pollen allergen.

Menz G(1), Dolecek C, Schönheit-Kenn U, Ferreira F, Moser M, Schneider T, Suter
M, Boltz-Nitulescu G, Ebner C, Kraft D, Valenta R.


8752951 J Immunol. 1996 Jul 15;157(2):956-62.

Human IgG monoclonal antibodies that modulate the binding of specific IgE to
birch pollen Bet v 1.

Visco V(1), Dolecek C, Denépoux S, Le Mao J, Guret C, Rousset F, Guinnepain MT,
Kraft D, Valenta R, Weyer A, Banchereau J, Labecque S.


8911702 Clin Exp Allergy. 1996 Oct;26(10):1161-70.

IgE cross-reactivity between birch pollen, mugwort pollen and celery is due to at
least three distinct cross-reacting allergens: immunoblot investigation of the
birch-mugwort-celery syndrome.

Bauer L(1), Ebner C, Hirschwehr R, Wüthrich B, Pichler C, Fritsch R, Scheiner O,
Kraft D.


8939154 J Allergy Clin Immunol. 1996 Nov;98(5 Pt 1):913-21.

Induction of IgE antibodies in mice and rhesus monkeys with recombinant birch
pollen allergens: different allergenicity of Bet v 1 and Bet v 2.

Vrtala S(1), Mayer P, Ferreira F, Susani M, Sehon AH, Kraft D, Valenta R.


9095250 Adv Exp Med Biol. 1996;409:251-4.

Characterisation of recombinant isoforms of birch pollen allergen Bet v 1.

Spangfort MD(1), Ipsen H, Sparholt SH, Aasmul-Olsen S, Osmark P, Poulsen FM,
Larsen M, Mørtz E, Roepstorff P, Larsen JN.


9011868 Pneumologie. 1996 Sep;50(9):632-40.

[Serologic and skin test diagnosis of birch pollen allergy with recombinant Bet v
1, the chief allergen of birch].

[Article in German]

Menz G(1), Dolecek C, Schönheit-Kenn U, Ferreira F, Moser M, Schneider T, Suter
M, Boltz-Nitulescu G, Ebner C, Kraft D, Valenta R.


9058694 J Allergy Clin Immunol. 1997 Mar;99(3):374-84.

Immunologic characterization of monoclonal antibodies that modulate human IgE
binding to the major birch pollen allergen Bet v 1.

Lebecque S(1), Dolecek C, Laffer S, Visco V, Denépoux S, Pin JJ, Guret C,
Boltz-Nitulescu G, Weyer A, Valenta R.


9116499 Protein Expr Purif. 1997 Feb;9(1):33-9.

High-level expression and purification of the major birch pollen allergen, Bet v


Hoffmann-Sommergruber K(1), Susani M, Ferreira F, Jertschin P, Ahorn H, Steiner
R, Kraft D, Scheiner O, Breiteneder H.


9130500 Int Arch Allergy Immunol. 1997 May-Jul;113(1-3):125-7.

Isoforms of atopic allergens with reduced allergenicity but conserved T cell
antigenicity: possible use for specific immunotherapy.

Ferreira F(1), Hirthenlehner K, Briza P, Breiteneder H, Scheiner O, Kraft D,
Breitenbach M, Ebner C.


9393965 Mol Immunol. 1997 Jun;34(8-9):619-29.

Molecular cloning, expression and characterization of Pru a 1, the major cherry
allergen.

Scheurer S(1), Metzner K, Haustein D, Vieths S.


9314348 J Allergy Clin Immunol. 1997 Sep;100(3):356-64.

Natural latex, grass pollen, and weed pollen share IgE epitopes.

Fuchs T(1), Spitzauer S, Vente C, Hevler J, Kapiotis S, Rumpold H, Kraft D,
Valenta R.


9472988 FASEB J. 1998 Feb;12(2):231-42.

Modulation of IgE reactivity of allergens by site-directed mutagenesis: potential
use of hypoallergenic variants for immunotherapy.

Ferreira F(1), Ebner C, Kramer B, Casari G, Briza P, Kungl AJ, Grimm R,
Jahn-Schmid B, Breiteneder H, Kraft D, Breitenbach M, Rheinberger HJ, Scheiner O.


9824411 Clin Exp Allergy. 1998 Nov;28(11):1384-8.

Genetic and environmental factors affecting the allergenicity of birch (Betula
pubescens ssp. czerepanovii [Orl.] Hämet-ahti) pollen.

Ahlholm JU(1), Helander ML, Savolainen J.


9802365 J Allergy Clin Immunol. 1998 Oct;102(4 Pt 1):579-91.

Recombinant birch pollen allergens (rBet v 1 and rBet v 2) contain most of the
IgE epitopes present in birch, alder, hornbeam, hazel, and oak pollen: a
quantitative IgE inhibition study with sera from different populations.

Niederberger V(1), Pauli G, Grönlund H, Fröschl R, Rumpold H, Kraft D, Valenta R,
Spitzauer S.


10380779 Allergy. 1999 May;54(5):478-83.

Immunologic significance of respirable atmospheric starch granules containing
major birch allergen Bet v 1.

Schäppi GF(1), Taylor PE, Staff IA, Rolland JM, Suphioglu C.


10502033 Eur J Nutr. 1999 Aug;38(4):201-15.

Pollen-related food allergy: cloning and immunological analysis of isoforms and
mutants of Mal d 1, the major apple allergen, and Bet v 1, the major birch pollen
allergen.

Son DY(1), Scheurer S, Hoffmann A, Haustein D, Vieths S.


10509815 Mol Immunol. 1999 Jul;36(10):639-45.

New Bet v 1 isoforms including a naturally occurring truncated form of the
protein derived from Austrian birch pollen.

Friedl-Hajek R(1), Radauer C, O'Riordain G, Hoffmann-Sommergruber K, Leberl K,
Scheiner O, Breiteneder H.


10629461 J Allergy Clin Immunol. 2000 Jan;105(1 Pt 1):116-25.

Quantitative IgE inhibition experiments with purified recombinant allergens
indicate pollen-derived allergens as the sensitizing agents responsible for many
forms of plant food allergy.

Kazemi-Shirazi L(1), Pauli G, Purohit A, Spitzauer S, Fröschl R,
Hoffmann-Sommergruber K, Breiteneder H, Scheiner O, Kraft D, Valenta R.


10877820 FASEB J. 2000 Jul;14(10):1279-88.

Rapid production of the major birch pollen allergen Bet v 1 in Nicotiana
benthamiana plants and its immunological in vitro and in vivo characterization.

Krebitz M(1), Wiedermann U, Essl D, Steinkellner H, Wagner B, Turpen TH, Ebner C,
Scheiner O, Breiteneder H.


10931114 Clin Exp Allergy. 2000 Aug;30(8):1076-84.

Comparison of genetically engineered hypoallergenic rBet v 1 derivatives with
rBet v 1 wild-type by skin prick and intradermal testing: results obtained in a
French population.

Pauli G(1), Purohit A, Oster JP, De Blay F, Vrtala S, Niederberger V, Kraft D,
Valenta R.


11307026 Int Arch Allergy Immunol. 2001 Jan-Mar;124(1-3):395-7.

IgE mimotopes of birch pollen allergen Bet v 1 induce blocking IgG in mice.

Ganglberger E(1), Grünberger K, Wiedermann U, Vermes M, Sponer B, Breiteneder H,
Scheiner O, Boltz G, Jensen-Jarolim E.


11932495 Protein Eng. 2002 Mar;15(3):243-9.

A recombinant bacterial cell surface (S-layer)-major birch pollen allergen-fusion
protein (rSbsC/Bet v1) maintains the ability to self-assemble into regularly
structured monomolecular lattices and the functionality of the allergen.

Breitwieser A(1), Egelseer EM, Moll D, Ilk N, Hotzy C, Bohle B, Ebner C, Sleytr
UB, Sára M.


12218371 Int Arch Allergy Immunol. 2002 Aug;128(4):325-35.

Different IgE reactivity profiles in birch pollen-sensitive patients from six
European populations revealed by recombinant allergens: an imprint of local
sensitization.

Movérare R(1), Westritschnig K, Svensson M, Hayek B, Bende M, Pauli G, Sorva R,
Haahtela T, Valenta R, Elfman L.


14720271 Clin Exp Allergy. 2004 Jan;34(1):115-22.

Vaccines for birch pollen allergy based on genetically engineered hypoallergenic
derivatives of the major birch pollen allergen, Bet v 1.

Mahler V(1), Vrtala S, Kuss O, Diepgen TL, Suck R, Cromwell O, Fiebig H, Hartl A,
Thalhamer J, Schuler G, Kraft D, Valenta R.


16272319 J Immunol. 2005 Nov 15;175(10):6645-50.

Dimerization of the major birch pollen allergen Bet v 1 is important for its in
vivo IgE-cross-linking potential in mice.

Schöll I(1), Kalkura N, Shedziankova Y, Bergmann A, Verdino P, Knittelfelder R,
Kopp T, Hantusch B, Betzel C, Dierks K, Scheiner O, Boltz-Nitulescu G, Keller W,
Jensen-Jarolim E.


17912007 Int Arch Allergy Immunol. 2008;145(3):193-206. Epub 2007 Oct 2.

Characterization of a hypoallergenic recombinant Bet v 1 variant as a candidate
for allergen-specific immunotherapy.

Kahlert H(1), Suck R, Weber B, Nandy A, Wald M, Keller W, Cromwell O, Fiebig H.


17979095 Mol Nutr Food Res. 2007 Dec;51(12):1527-36.

Purification and characterization of natural Bet v 1 from birch pollen and
related allergens from carrot and celery.

Bollen MA(1), Garcia A, Cordewener JH, Wichers HJ, Helsper JP, Savelkoul HF, van
Boekel MA.


18268388 Int Arch Allergy Immunol. 2008;146(3):203-11. doi: 10.1159/000115888. Epub 2008
Feb 11.

Purification and characterization of the major oak pollen allergen Que a 1 for
component-resolved diagnostics using ImmunoCAP.

Movérare R(1), Everberg H, Carlsson R, Holtz A, Thunberg R, Olsson P, Brostedt P,
Hogbom E.


19183416 Allergy. 2009 Jul;64(7):1038-45. doi: 10.1111/j.1398-9995.2009.01957.x. Epub 2009
Jan 31.

Standardization of allergen products: 1. Detailed characterization of
GMP-produced recombinant Bet v 1.0101 as biological reference preparation.

Himly M(1), Nony E, Chabre H, Van Overtvelt L, Neubauer A, van Ree R, Buchheit
KH, Vieths S, Moingeon P, Ferreira F.


20447079 Clin Exp Allergy. 2010 Apr;40(4):679-87. doi: 10.1111/j.1365-2222.2010.03486.x.

Identification of Bet v 1-related allergens in fig and other Moraceae fruits.

Hemmer W(1), Focke M, Marzban G, Swoboda I, Jarisch R, Laimer M.


21215346 Vaccine. 2011 Mar 3;29(11):2140-8. doi: 10.1016/j.vaccine.2010.12.080. Epub 2011
Jan 5.

Genetic engineering of trimers of hypoallergenic fragments of the major birch
pollen allergen, Bet v 1, for allergy vaccination.

Vrtala S(1), Fohr M, Campana R, Baumgartner C, Valent P, Valenta R.


21459169 J Proteomics. 2011 Aug 12;74(8):1290-300. doi: 10.1016/j.jprot.2011.03.021. Epub
2011 Apr 1.

Proteomic analysis of the major birch allergen Bet v 1 predicts allergenicity for
15 birch species.

Schenk MF(1), Cordewener JH, America AH, Peters J, Smulders MJ, Gilissen LJ.


--DOI:10.1186/2045-7022-3-S3-O15
27770477 Clin Exp Allergy. 2017 May;47(5):693-703. doi: 10.1111/cea.12835. Epub 2016 Nov


IgE and allergen-specific immunotherapy-induced IgG4 recognize similar epitopes
of Bet v 1, the major allergen of birch pollen.

Groh N(1), von Loetzen CS(2), Subbarayal B(3), Möbs C(4), Vogel L(1), Hoffmann
A(1), Fötisch K(1), Koutsouridou A(1), Randow S(1), Völker E(1), Seutter von
Loetzen A(1), Rösch P(2), Vieths S(1), Pfützner W(4), Bohle B(3), Schiller D(1).


30706562 Clin Exp Allergy. 2019 May;49(5):712-723. doi: 10.1111/cea.13356. Epub 2019 Feb


Quality and potency profile of eight recombinant isoallergens, largely mimicking
total Bet v 1-specific IgE binding of birch pollen.

Seutter von Loetzen C(1), Reuter A(2), Spiric J(2), Schulenborg T(2),
Bellinghausen I(3), Völker E(2), Vogel L(2), Rösch P(1), Schiller D(2).


32375962 Allergy Asthma Proc. 2020 May 1;41(3):183-191. doi: 10.2500/aap.2020.41.200002.

Serum immunoglobulin E reactivity to cross-reacting panallergen components in
north-eastern Poland patients pollen sensitized.

Kowal K(1), Pampuch A(2), Sacharzewska E(1), Swiebocka E(3), Siergiejko Z(4),
Siergiejko G(5), Chruszcz M(6), DuBuske L(7).


32784509 Int J Mol Sci. 2020 Aug 8;21(16). pii: E5693. doi: 10.3390/ijms21165693.

Filling the Antibody Pipeline in Allergy: PIPE Cloning of IgE, IgG1 and IgG4
against the Major Birch Pollen Allergen Bet v 1.

Köhler VK(1)(2), Crescioli S(3)(4), Fazekas-Singer J(1)(2), Bax HJ(3)(5), Hofer
G(6), Pranger CL(1)(2), Hufnagl K(1)(2), Bianchini R(1)(2), Flicker S(2), Keller
W(6), Karagiannis SN(3)(4)(7), Jensen-Jarolim E(1)(2).